abstract：:The bioassay system from American Diagnostic for amikacin was compared to the same company's radioimmunoassay (RIA) technique used in this hospital. The bioassay is performed by adding serum from a patient to wells cut in agar which have been seeded with a susceptible fast growing microorganism (Enterobacter). After i...

journal_title：Annals of clinical and laboratory science

authors： Manos JP,Botting AJ,Burdash NM,Mason MH,West ME

更新日期：1981-09-01 00:00:00

abstract：:A simple, fast, reproducible, and reliable fibrometer method for determination of plasma or serum plasminogen was developed. Results obtained with this method were compared to those obtained with a Coseinolytic method and a Chromogenic Substrate S-2251 method. For all three procedures, serum or plasma samples were aci...

journal_title：Annals of clinical and laboratory science

authors： Sarji NM,Liu PI,Teaford MJ,Crook L

更新日期：1981-09-01 00:00:00

abstract：:A retrospective study of acquired renal lesions in 42 renal transplants in children revealed a variety of histologic lesions. Although acute and chronic rejection changes were the most frequent, a number of metabolic, infectious, vascular, and other lesions were encountered. Renal biopsy allows rapid diagnosis of thes...

journal_title：Annals of clinical and laboratory science

authors： Wenzl JE,Krous HF

更新日期：1981-07-01 00:00:00

abstract：:Recent improvement in imaging technology has enabled ultrasound to assume an increasingly vital role in the identification and evaluation of renal disease. Renal masses can be differentiated. The nonfunctioning kidney can be imaged and obstruction or inflammation diagnosed. When further information is needed, an ultra...

journal_title：Annals of clinical and laboratory science

authors： Wilson DA

更新日期：1981-07-01 00:00:00

abstract：:The possible pathologic consequences of the presence of circulating immune complexes in certain disease states has caused considerable attention to be given to the development of laboratory procedures capable of quantitatively measuring the presence of immune complexes in serum. Many assays are available which utilize...

journal_title：Annals of clinical and laboratory science

authors： Blackstock R

更新日期：1981-05-01 00:00:00

abstract：:Alterations in ion transport have been implicated in the pathogenesis of several disease states. Methods of studying ion transport, however, are rather tedious, time consuming and not well defined in terms of kinetics and reproducibility. This paper describes a rapid, simple method of examining ion transport in intact...

journal_title：Annals of clinical and laboratory science

authors： Michels RC,Ober KP,Hennessy JF

更新日期：1981-03-01 00:00:00

abstract：:The ability of three isolates of serogroup 1 and one isolate of serogroup 4 of Legionnaires' disease bacterium (LDB) to infect and cause fever and death in guinea pigs was studied, as well as their ability to produce plaques in cultured primary chick embryo cells. The serogroup 4 isolate originally was recovered from ...

journal_title：Annals of clinical and laboratory science

authors： Ormsbee RA,Peacock MG,Bickel WD,Fiset P

更新日期：1981-01-01 00:00:00

abstract：:Although serum bilirubin determination is a common procedure in most clinical laboratories, the test seems to be of particular importance in the screening of neonates, especially the premature or the erythroblastotic infant. However, bilirubin determination is subject to interferences, particularly in the presence of ...

journal_title：Annals of clinical and laboratory science

authors： Baginski ES,Epstein E,Zak B

更新日期：1980-11-01 00:00:00

abstract：:Methodologies for T and B lymphocyte quantitation, lymphocyte blast transformation (LBT) and carbohydrate (CHO) metabolism are important for assessing host lymphocyte response in the clinical laboratory. Modifications of methods for each of these techniques are presented. Results from studies of normal ambulatory adul...

journal_title：Annals of clinical and laboratory science

authors： Glassman AB,Bennett CE

更新日期：1980-11-01 00:00:00

abstract：:The anucleate mature erythrocyte also lacks ribosomes and mitochondria and thus cannot synthesize enzymes or derive energy from the Krebs citric acid cycle. Nevertheless, the red blood cell is metabolically active and contains numerous residual enzymes and their products which are essential for its survival and normal...

journal_title：Annals of clinical and laboratory science

authors： Netzloff ML

更新日期：1980-09-01 00:00:00

abstract：:A rapid homogeneous enzyme immunoassay for theophylline has been adapted to a centrifugal analyzer. The sample volume used was 10 microliter and sera from as many as 28 patients could be measured in 180 seconds. The assay temperature was 30 degrees and the absorbance rates were measured at 340 nm. Within-run and run-t...

journal_title：Annals of clinical and laboratory science

authors： Lacher DA,Sinn JA,Savory J,Wills MR

更新日期：1980-07-01 00:00:00

abstract：:Erythropoietin is a hormone produced by the kidneys and by certain extrarenal tissues and released into the circulation in response to tissue hypoxia. Its study has provided new information about oxygen transport and bone marrow stem cell function and its determination in plasma can give valuable diagnostic clues as t...

journal_title：Annals of clinical and laboratory science

authors： Erslev AJ,Caro J,Miller O,Silver R

更新日期：1980-05-01 00:00:00

abstract：:Acanthocytosis represents an unusually pathological variant of red cell morphology which is encountered in a diverse group of inherited and acquired disease states. While the morphological features are similar in all instances, the biochemical lesions frequently differ. Most demonstrable abnormalities involve lipids a...

journal_title：Annals of clinical and laboratory science

authors： Biemer JJ

更新日期：1980-05-01 00:00:00

abstract：:HbA1 is the result of the non-enzymatic glycosylation of HbA. HbA1 reflects the integrated blood glucose level that prevailed over a period of weeks; it thus has clinical application in the assessment of the long-term blood glucose control. This ability to determine easily the long-term blood glucose should enable the...

journal_title：Annals of clinical and laboratory science

authors： Rochman H

更新日期：1980-03-01 00:00:00

abstract：:A high performance liquid chromatography has been adapted to the measurement of putrescine, spermidine and spermine in plasma and erythrocytes. Detection was by fluorimetry using o-phthalaldehyde which formed fluorophores with the polyamines. Sensitivity of the method was better than 10 pmol of each polyamine, and rec...

journal_title：Annals of clinical and laboratory science

authors： Shipe JR Jr,Savory J

更新日期：1980-03-01 00:00:00

abstract：:Secretion of nickel into unstimulated and stimulated saliva of rabbits was studied after a single intraperitoneal injection of nickel chloride labelled with 63Ni. Maximum 63Ni in unstimulated saliva was obtained in specimens collected one hr after maximum serum-63Ni was reached and two hrs after the nickel administrat...

journal_title：Annals of clinical and laboratory science

authors： Hofsøy H,Paulsen G,Jonsen J

更新日期：1979-11-01 00:00:00

abstract：:The histopathologic findings in lung tissue are reported from five cases of Philadelphia Legionnaire's Disease and the results are compared to pneumonias caused by other microbial and chemical agents. Histopathology of lung tissue was similar in all cases, despite the fact that death occurred between the fourth and 14...

journal_title：Annals of clinical and laboratory science

authors： Lattimer GL,Rachman RA,Scarlato M

更新日期：1979-09-01 00:00:00

abstract：:Malignant tumours of nonendocrine tissues may produce ectopic hormones. The most likely mechanism is depression of genes which code for hormones. Ectopic hormones are invariably peptides, and each is identical to some peptide product of an endocrine gland. However, the majority of ectopic hormones occur as biologicall...

journal_title：Annals of clinical and laboratory science

authors： Hollander IJ,Aponte GE

更新日期：1979-07-01 00:00:00

abstract：:Tissue obtained from 55 men with prostatic disease was assayed for estrogen and androgen receptors by a newly developed histochemical technique. The material studied consisted of 45 specimens of benign nodular prostatic hyperplasia and 10 specimens of prostatic adenocarcinoma. The results obtained were compared to tho...

journal_title：Annals of clinical and laboratory science

authors： Pertschuk LP,Zava DT,Gaetjens E,Macchia RJ,Wise GJ,Kim DS,Brigati DJ

更新日期：1979-05-01 00:00:00

abstract：:The classification of ovarian tumors presents a difficult problem because of the great variety of tumor types that can occur. The complex structure of the normal ovary and the diversity of cell types present at different stages of development contribute to this difficulty. Developmental and ultrastructural studies hav...

journal_title：Annals of clinical and laboratory science

authors： Gondos B

更新日期：1979-05-01 00:00:00

abstract：:The most common ultrastructural features of endocrine tumors are reviewed. The diagnostic applications of the electron microscope are illustrated by citation of case histories. ...

journal_title：Annals of clinical and laboratory science

authors： Battifora HA

更新日期：1979-03-01 00:00:00

abstract：:Male Swiss Webster mice were fed a diet containing 2.5 percent griseofulvin (GF). Serially progressive light and electron microscopic hepatic altertions were present from one day on and grossly visible hepatomegaly from two weeks until termination at 22 weeks. GF induced liver changes included hepatocyte nuclear and c...

journal_title：Annals of clinical and laboratory science

authors： Wessely Z,Shapiro SH,Klavins JV

更新日期：1979-01-01 00:00:00

abstract：:This is a review of the following human gene products which appear in the prenatal period, subsequently are repressed, and reappear in malignant neoplasms: alpha fetal protein; carcinoembryonic antigen; Regan's isoenzyme; fetal sulfoglycoprotein; a fetal liver ferroprotein; beta-S-fetoprotein; gamma-fetoprotein; carci...

journal_title：Annals of clinical and laboratory science

authors： Klavins JV

更新日期：1978-09-01 00:00:00

abstract：:Macrophage-migration inhibition factor (MIF) is a lymphocyte-derived substance which plays an important role in cell mediated immunity. Soluble factors containing MIF-like activity and produced by non-stimulated and virus-infected non-lymphoid cell cultures have also been reported. In the present study, a MIF-like fac...

journal_title：Annals of clinical and laboratory science

authors： Brown EW,Burdash NM,Manos JP,Duncan RC

更新日期：1978-09-01 00:00:00

abstract：:A brief historical review is presented of the development of bovine serum albumin as a standard for the determination of serum total protein. Bovine serum albumin is now available as a standard reference material from the National Bureau of Standards. Also reviewed is the present status of the immunoglobulin standards...

journal_title：Annals of clinical and laboratory science

authors： Klein B

更新日期：1978-05-01 00:00:00

abstract：:A case of multiple myeloma producing IgD-M component with light chains of lambda type is reported. It involved the skeleton and was complicated by compression of the spinal cord and massive extension to the pleura. The urine contained lambda Bence Jones protein. Under the electron microscope, the neoplastic plasma cel...

journal_title：Annals of clinical and laboratory science

authors： Ioachim NJ,McKenna PJ,Halperin I,Chung FJ

更新日期：1978-05-01 00:00:00

abstract：:Fractionation of serum alkaline phosphatase by either biochemical or electrophoretic techniques is often insufficient to determine the source of the elevated serum enzyme. In 31 unselected patients with high serum alkaline phosphatase a simultaneous determination of serum gamma glutamyl transpeptidase and urinary hydr...

journal_title：Annals of clinical and laboratory science

authors： Slaunwhite D,Tuggey RL,Reynoso G

更新日期：1978-03-01 00:00:00

abstract：:A series of studies were undertaken to evaluate the chemotherapeutic response to various antineoplastic drugs of human breast (MX-1) or colon (CX-2) tumor xenografts growing in genetically athymic (nude) mice. Fragments (2mm3) of either tumor type were implanted subcutaneously into the subaxillary region of NIH Swiss ...

journal_title：Annals of clinical and laboratory science

authors： Ovejera AA,Houchens DP,Barker AD

更新日期：1978-01-01 00:00:00

abstract：:The presence of effusion in association with multiple myeloma or macroglobulinemia is an unusual finding which generally occurs late in the course of the disease. Occasionally, cytoligic detection is a diagnostic problem. In both conditions, the fluid is characrerized by a high specific gravity and high protein conten...

journal_title：Annals of clinical and laboratory science

authors： Gondos B,Miller TR,King EB

更新日期：1978-01-01 00:00:00

abstract：:A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previou...

journal_title：Annals of clinical and laboratory science

authors： Baginski ES,Marie SS,Epstein E,Zak B

更新日期：1977-11-01 00:00:00

abstract：:In vitro activity of erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) does not always correlate with in vivo hemolytic manifestations. Many of these non-correlations are reviewed and can now be explained on the basis of altered substrate affinity and/or altered inhibition by intracellular metabolites. These alte...

journal_title：Annals of clinical and laboratory science

authors： Brewster MA,Quittner H,Moriarity M

更新日期：1977-07-01 00:00:00

abstract：:Blood lead levels were measured by atomic absorption spectrophotometry in 86 San Diego policemen to see if they would reflect the known high atmospheric lead levels in San Diego. The mean blood lead for the entire group was 27.0 microgram per dl, a level very similar to that found in the general population of other ci...

journal_title：Annals of clinical and laboratory science

authors： Zettner A,Josselson AR,Ramras DG,Askew JB

更新日期：1977-07-01 00:00:00

abstract：:Renal arterioles from five patients with benign essential hypertension (BH) and four patients with malignant essential hypertension (MH) were studied using electron microscopy (EM). This study suggests a concept of different pathogenetic mechanism for renal vascular (arteriolar) lesions in BH and MH (i.e., benign and ...

journal_title：Annals of clinical and laboratory science

authors： Mandal AK,Frohlich ED,Nordquist J

更新日期：1977-03-01 00:00:00

abstract：:Erythrocytosis was induced in Fischer rats by intrarenal (ir) injection of nickel subsulfide (Ni3S2) in dosages ranging from 0.6 to 10 mg per rat. Measurements of blood packed cell volume (PCV) became increased by one month after ir injection of Ni3S2; reached maximum values at approximately two months; and gradually ...

journal_title：Annals of clinical and laboratory science

authors： Morse EE,Lee TY,Reiss RF,Sunderman FW Jr

更新日期：1977-01-01 00:00:00

abstract：:Numerous drugs and chemicals affect the function of human blood platelets. The mechanism of action of some medications is partly understood. Aspirin is the most frequently involved drug. It appears to interfere with the platelet release reaction by acetylation of a platelet membrane protein which may be involved in th...

journal_title：Annals of clinical and laboratory science

authors： Morse EE

更新日期：1977-01-01 00:00:00

abstract：:Widespread drug abuse, a comparatively recent medicosocial phenomenon, presents protean clinical patterns and challenging diagnostic problems daily that mimic classical medical syndromes. However, few reports have delineated predominantly neurologic syndromes associated with drug abuse. Five patients were observed ill...

journal_title：Annals of clinical and laboratory science

authors： Stevens H,Restak R

更新日期：1976-11-01 00:00:00

abstract：:A statistically significant difference was found between the mean baseline carboxyhemoglobin of non-smoking firemen (COHb 2.53, sd = 1.05, n = 32) and smoking firemen (COHb 5.54, sd = 2.61, n = 26). A consistent increase in mean COHb levels after exposure to smoke was seen in both non-smoking (4.41 sd = 2.95, n = 7) a...

journal_title：Annals of clinical and laboratory science

authors： Levy AL,Lum G,Abeles FJ

更新日期：1976-09-01 00:00:00

abstract：:A procedure for the measurement of free erythrocyte protoporphyrin (FEP) in a drop of blood collected on filter paper is described. The method is useful as a screening test for lead poisoning in children. Based on the FEP finding and blood lead tests, asymptomatic children are classified into four major categories. A ...

journal_title：Annals of clinical and laboratory science

authors： Davidow B,Slavin G,Piomelli S

更新日期：1976-05-01 00:00:00

abstract：:Until recently it has been generally accepted that the choroid plexuses are the major source of the cerebrospinal fluid (CSF). However, data now indicate that significant contributions to the CSF are made at extrachoroidal sites. CSF production studies and 24Na transport studies in normal and plexectomized rhesus monk...

journal_title：Annals of clinical and laboratory science

authors： Hammock MK,Milhorat TH

更新日期：1976-01-01 00:00:00

abstract：:The conjugated bile acid pattern was evaluated in intestinal aspirates of five normal subjects and five patients known to have an abnormal bile acid distribution. The glycine/taurine (G/T) ratios for total bile acids were determined by gas liquid chromatography (GLC) and by enzymatic assay using 3-hydroxysteroid dehyd...

journal_title：Annals of clinical and laboratory science

authors： Haeffner LJ,Gordon SJ,Strum S,Elliot M,Kowlessar OD

更新日期：1976-01-01 00:00:00